منابع مشابه
Shotgun protein sequencing with meta-contig assembly.
Full-length de novo sequencing from tandem mass (MS/MS) spectra of unknown proteins such as antibodies or proteins from organisms with unsequenced genomes remains a challenging open problem. Conventional algorithms designed to individually sequence each MS/MS spectrum are limited by incomplete peptide fragmentation or low signal to noise ratios and tend to result in short de novo sequences at l...
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Despite the steady accumulation of fully sequenced genomes for model organisms, limited or no sequence information is available for most organisms. Moreover, natural mechanisms of variation such as accelerated mutation and combinatorial recombination in immunoglobulins regularly create novel sequences in the proteomes of model organisms. However, since protein identification via database search...
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Although snake venom proteins have been proven instrumental in the design of blood clotting and cytostatic breast cancer drugs, the main method in use to sequence these unknown proteins is still the low-throughput process of Edman degradation. Moreover, the common approach to MS/MS protein sequence identification is limited in that it focuses on the identification of single MS/MS spectra from m...
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Large-scale sequencing of the human genome is now under way (Boguski et al. 1996; Marshall and Pennisi 1996). Although at the beginning of the Genome Project, many doubted the scientific value of sequencing the entire human genome, these doubts have evaporated almost entirely (Gibbs 1995; Olson 1995). Primary reasons for generating the human genomic sequence are listed in Table 1. The approach ...
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Shotgun sequencing is the most powerful strategy for large scale sequencing. Two main approaches exist: clone-by-clone and whole genome shotgun (WGS). In the clone-by-clone strategy, overlapping clones are amplified and then sheared in a random fashion. In the WGS approach, a sufficient amount of cells from the target organism are obtained, and the random shearing is performed on extracted DNA....
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2007
ISSN: 1535-9476
DOI: 10.1074/mcp.m700001-mcp200